1135 Evaluation of a method for trabecular meshwork perfusion of half anterior segments of the human eye

&gl~$g. Anterior segment perfusion povides a tool for determining flow in the trabecular outflow pathway only, contrary to perfusion of whole eyes in which 10-3046 of the outflow comes on account of the uveoscleral pathway. Since disposed human eyes are not abundantly available, especially not as a pair, it would be convenient to develop a model in which one half of an anterior segment could serve as a control for the other, drug treated half. It would also bc of great advantage in blocker studies. In this study we report a new method to determine flow in half anterior segments. &~&JR& A chamber was developed to handle half anterior segments.The chamber provides a maximum tit and a minimum of stress on the shape of the essential structures. Several parameters were evaluated to compare whole with half segments. Also some drugs were applied. Real time flow data were collected for later analysis &J.&. It is possible to divide anterior segments for flow measurement. Some flow is lost due to clamping. Flow seems to be unequally distributed through the meshwork, indicating areas of preferential flow. Drug effects were established in the presence of a control. Conclusion. Perfusion of half segments offer a new perspective to the study of the trabecular meshwork. More investieations will be done in order to further establish the usefulness and applicability of this technique.